Criteria for Probiotic Classification (32)

For a probiotic to be effective, it must:
1. Resist gastric, bile, and pancreatic digestion
2. Adhere to the mucosal and epithelial surfaces
3. Once colonized, remain metabolically active
4. Provide the host with protection from pathogens by secreting antimicrobial substances or preventing their adhesion and colonization
5. Demonstrate bile salt hydrolase activity